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KMID : 0615720090260050065
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Jouranl of Korean Acupuncture and Moxibustion society
2009 Volume.26 No. 5 p.65 ~ p.75
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Effects of Administration of Pyritum on Fracture Healing in Mice
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Shin Kyung-Min
Kim Kap-Sung
Hwang Min-Seop
Kim Kyung-Ho
Lee Seung-Deok
Jung Chan-Yung
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Abstract
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Objectives : Pyrite is one of the important prescriptions that has been used in oriental medicine for healing of fracture. It is reasonable, therefore, to postulate that native copper affects the process of bone metabolism and bone formation. The purpose of this study is to discover the effect of Pyrite on the healing of tibia fracture.
Methods : 1. In vitro test : MG-63 cell in human body and the Pyritum in the ratio of 0.5mg/ml, 1.0mg/ml, 1.5mg/ml, 2.0mg/ml were incubated for 24 hours. After 24 hours, RNA was extracted via trizol reagent (Sigma, USA). In order to understand the activation of osteoblast, the level of OPN mRNA, osteopontin, was measured. 2. In vivo tesgroups normal group, control group and experimental group. Left tibia bones of mice in CON and JT groups were fractured by bone cutters. Pyrite was orally administered to the experimental group. After 14 days, each group’s tibia specimen was constructed to observe changes in activation of proinflmmatory cytokines in relation to MIF and IL-6. Also, proliferation of osteoblast and osteopontin were measured via changes in levels of OPN and OPN mRNA.
Results : In in-vitro test, the level of OPN mRNA, osteopontin production was remarkably increased in Pyritum-treated MG-63 cells. In in-vivo test, fractured area in external tibia morphology was increased more in the JT group than that of the CON group. Osteogenesis, endochodrial ossification, and osteoid in fractured area were also increased more in the JT group than that of the CON group. Increase in OPN mRNA, osteopontin level and osteoblast’s proliferation were observed. Activation of MIF and IL-6 was confirmed from the fracture region.
Conclusions : From the result, development of a new stimulator in healing fracture via pyrite is expected.
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KEYWORD
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Pyrite, Pyritum, native Copper, osteoblast, bone fracture
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